Polymorphism in the promoter of TLR4 gene by PCR-RFLP and its association with somatic cell score in Chinese Holstein
نویسندگان
چکیده
The objective of this study was to examine the effect of the polymorphism in the promoter of toll-like receptor 4 (TLR4) on mastitis and milk production traits. Two novel single nucleotide polymorphisms (SNPs) G-1539A before 5’-upstream region and G+265C in the 5’-UTR of TLR4 gene were detected, and genetic association analysis was applied on 421 Chinese Holstein cows. The statistical analysis indicated that two SNPs was significantly associated with 305 Days milk yield (P<0.05) and somatic cell score (SCS) respectively (P<0.01). Our results provide evidence that polymorphisms in the promoter of TLR4 are associated with milk production traits and SCS, and may be used as a possible candidate for marker-assisted selection and management in Holstein cattle breeding program.
منابع مشابه
Single Nucleotide Polymorphism in the Promoter Region of Bovine Interleukin 8 Gene and its Association with Milk Production Traits and Somatic Cell Score of Holstein Cattle in Iran
Background: Interleukie-8 is a proinflammatory cytokine and a potent chemotactic and activation factor for neutrophils. It has been reported to be expressed in high levels by various cell types after immune stimulation and mediates neutrophil recruitment in host. Objectives: The aim of this study was to analysis of the relationship between single nucleotide polymorphisms (SNPs) at position -18...
متن کاملInfluence of the Bovine Acyl-Coa: Diacylglycerol Acyltransferase1 (DGAT1) K232A on Milk Production and Somatic Cell Score Holstein Cows
This experiment was aimed to study the association between the DGAT1 K232A polymorphism and milk production traits and somatic cell score (SCS) in Iranian Holstein dairy cows. The records of 408 animals from five dairy herds were randomly identified and then genomic DNA was extracted from blood using the modified-salting method described by Miller. RFLP-PCR was performed to obtain all the polym...
متن کاملInfluence of the Bovine Acyl-Coa: Diacylglycerol Acyltransferase1 (DGAT1) K232A on Milk Production and Somatic Cell Score Holstein Cows
This experiment was aimed to study the association between the DGAT1 K232A polymorphism and milk production traits and somatic cell score (SCS) in Iranian Holstein dairy cows. The records of 408 animals from five dairy herds were randomly identified and then genomic DNA was extracted from blood using the modified-salting method described by Miller. RFLP-PCR was performed to obtain all the polym...
متن کاملSingle nucleotide polymorphism of the lactoferrin gene and its association with milk production and reproduction traits in Iranian Holstein cattle
Bovine lactoferrin (LTF) is a member of the transferrin family of iron-binding proteins. This protein is present in a wide variety of biological fluids and shows important physiological functions in body. In this study, 404 blood samples were collected from Holstein dairy farms in Iran. A 301 bp fragment of intron 6 in bovine LTF gene was amplified and the animals were genotyped using polymeras...
متن کاملPolymorphism in PGLYRP-2 gene by PCR-RFLP and its association with somatic cell score and percentage of fat in Chinese Holstein.
Peptidoglycan recognition protein 2 (PGLYRP-2), which belongs to the PGRP family, is the only member that has no direct bactericidal activity but has N-acetylmuramoyl-l-alanine amidase activity. This feature of PGLYRP-2 indicates that it may play an important role in eliminating the pathogen associated molecular pattern (PAMP), such as peptidoglycan (PGN), which can reduce leukocytes in blood a...
متن کامل